HOTSON. — CULTURE STUDIES OF FUNGI. 257 



to, there were also found associated with the " Harzia-like" fructifica- 

 tion, chlamydospores which are ovoid, smooth, brownish, thick- 

 walled, and have the distal end rounded. They are produced usually 

 on short lateral branches which taper towards the tips and may be 

 continuous or septate. The mature spores are quite uniform in size, 

 about 17 X 21 n, although there were some that appeared to be mature, 

 which were slightly smaller than this. These spores resemble both 

 in color and form those of Acremoniella atra Sacc. There are certain 

 other fungi that produce imperfect forms of the "Harzia" and 

 "Acremoniella" type which will he further considered below in 

 connection with P. aspergilliformis. 



Melanospora anomala n. sp. 

 Plate 2, Figures 27-30; Plate 3, Figures 1-15. 



Perithecia scattered or gregarious, superficial membranous, straw- 

 colored or light brown, globose or subglobose, 250-350^X350- 

 450 ii, ostiole formed in connection with a definite but inconspicuous 

 papilla without setae, primordium a spiral of 4 or 5 coils; ascospores 

 ;i mmetrical, somewhat crescent-shaped 14 X 28 ju, yellowish brown 

 becoming brownish black; conidia, hyaline, spherical to ovoid, on 

 flask-shaped sterigmata: bulbils yellowish brown, variable in size 

 70-140 ji in diameter, sometimes elongated ones 180 /u in length, 

 primordium a group of intercalary cells. 



On Spanish chestnuts in laboratory culture. 



Gross cultures of Spanish chestnuts, which were imported probably 

 from Spain obtained by the writer in the Boston market, produced 

 numerous brownish colored bulbils when cultivated in moist chambers. 

 By using the general methods already described, separate bulbils 

 were transferred to sterilized nutrient-agar tubes and, after a few 

 transfers, were obtained pure. 



The mycelium of this fungus is white and more or less aerial, vary- 

 ing according to the media in which it is grown. When grown on soft 

 chestnut agar, it becomes quite flocculent, while on chestnut decoc- 

 tion it forms a more or less felted layer over the surface, assuming the 

 brownish color of the liquid; but on potato agar its growth is rather 

 scanty. The diameter of the hyphae varies from 2.5-7 //. 



The bulbils. Scattered over the aerial hyphae and on the substra- 

 tum arc seen numerous small yellowish-brown bulbils, which, when 

 examined microscopically, are found to vary considerably in si/<- and 

 outline, many of them nearly spherical, others somewhat elongated. 



