HOTSOX. — CULTURE STUDIES OF FUNGI. 255 



of the cirri of ascospores and therefore probably pure. The writer 

 has under cultivation transfers of this same fungus but although it 

 has been grown on various kinds of media, both very rich and very 

 poor in nutrient material, and hard and soft, etc., yet thus far he has 

 not succeeded in obtaining either the chlamydospores or the " Harzia- 

 like" fructification. This is probably due to the fact that the pro- 

 duction of these structures is secured under certain peculiar conditions 

 not readily controlled. 



In general this fungus resembles M. papilluia in form and habit of 

 growth. The predominant type of reproduction in both is by asco- 

 spores the production of bulbils being scanty, while in some cases, as 

 on attenuated agar cultures, they are not produced at all. The peri- 

 theeium of M. cervicula which is usually 400-500 n in diameter, has a 

 definite neck 85-140 n in length, while M. papillata which is slightly 

 smaller, seldom reaching 500 n in diameter, has no neck but often 

 a papilla-like structure from which the setae arise. Moreover, the 

 former probably produces conidiophores of the "Harzia type" and 

 also chlamydospores which resemble those of Acremoniella atra. 



The Bulbils. — The mycelium is colorless, procumbent or only slightly 

 aerial, growing evenly over the surface of the substratum. The 

 hyphae, which are copiously septate, measure 5-7 n in diameter, but 

 often large swellings occur in them which seem to act as storage organs 

 and from which several branches may grow out as shown in Figure 21, 

 Plate 3. These arc found not infrequently on attenuated artificial 

 media such as agar alone without any nutriment, on which the mycel- 

 ium is very scanty, being barely visible even with the aid of a hand 

 lens. On such media, it should also be noted that as in .1/. papil- 

 lata, Bulbils are not produced. It further resembles the latter in 

 the mode of development of the bulbils, the primordium consisting of a 

 group of intercalary cells. It is, however, subject to considerably 

 ■greater variation and many irregular, incomplete or imperfect forms 

 appear. Since the mode of development is essentially the same as 

 that described for .1/. papillata, it will be unnecessary to repeat 

 the description here. They arc, however, produced very sparingly 

 on most media, and onsomi uchas that just mentioned, do nut occur 

 at all, although on a rich substratum not ton hard, such a 

 chestnul or bran agar they arc produced quite abundantly. 



The perithelium. — In i il the perithecium resembles thai of 



.V. papillata, but is clearly distinguished by having a definite neck. 

 They, however, arj considerably in size, sometime reaching 550 ju 

 in diameter, their form often b i omewhal contorted, with onlj 



