270 PROCEEDINGS OF THE AMERICAN' ACADEMY. 



verified. Lyman obtained his cultures from the basidiospores collected 



on old rotten oak logs in the field and pure cultures from these produced 

 bulbils. The writer began his cultures with bulbils, also collected 

 in the field, and, after a great number of unsuccessful attempts, finally 

 succeeded in obtaining a basidiosporic fructification similar to that 

 described by Lyman. This was accomplished by using gross cultures 

 of partly decayed wood in two litre Erlenmeyer flasks with sufficient 

 agar to hold them in place. The mycelium, as usual, produced bul- 

 bils profusely on the agar and wood, but after six or eight weeks near 

 the top of the pieces of wood conspicuous patches of white mycelium 

 appeared, which eventually produced the hymenium and basidiospores 

 of C. alutact urn. 



Papulospora anomala n. sp. 

 Plate 6, Figures 11-19. 



This form, which was obtained from four different localities, — 

 three from the vicinity of Claremont, California, found on Live Oak 

 chips, and one on an old paper from Cambridge. Mass., — has been 

 grown on a variety of substrata in the hope that it would produce its 

 perfect form, but thus far all these efforts have failed. That it belongs 

 to the Basidiomycetes is shown by its clamp-connections, which,' 

 however, are not so prominent as those in the two preceding forms, 

 from which it is further distinguished by the dark brown, opaque, 

 almost black color of the bulbils, the compact nature of their cells, 

 and their mode of development. The mycelium is white, procumbent, 

 scanty, slightly aerial on some substrata, with a large number of con- 

 spicuous oil globules, and not infrequently contains swollen intercalary 

 cells, which are also densely filled with food material and probably 

 act as storage organs. 



The bulbils. — The primary hyphae are small, seldom more than 

 3 /x in diameter, and do not produce bulbils; but scattered over the 

 secondary hyphae, which vary greatly in width, often reaching 10 n 

 and under some abnormal conditions 14//, are seen slightly swollen, 

 colorless, intercalary cells, quite different from those mentioned 

 above, about 4 or 5 yu in diameter, sometimes projecting considerably 

 and resembling short stunted branches; at other times the base of a 

 short lateral hypha swells slightly and forms the primordium (Figure 

 12, Plate 6). From the primordial cell or cells branches are sent out 

 in different directions, the basal cells of which become spherical and 

 in turn may produce other similar branches (Figures 13-15, Plate 6). 



