HOTSON. — CULTURE STUDIES OF FUNGI. 



269 



Grandinia has been produced cm gross wood cultures of this bulbil 

 and also on test-tube cultures of bran-agar of about 41) gm. of agar to 

 the litre, by three or four of the ten cultures from different sources 

 under cultivation. Preparatory to its formation, the mycelium ceases 

 to produce bulbils and forms a sort of incrustation, chalk-white in 

 color and becoming pustulate by the time the spores are funned, 

 Figure 1, Plate 6. The pustules on examination are found to be made 

 up of more or less thickly interwoven branching hyphae, which have 

 become enlarged and densely rilled with granular material and oil 

 globules, the ultimate ramifications of which form the hymenium 

 (Figure 2, Plate 6i. The basidia, which form a somewhat loose hy- 

 menium, each produce four spores, which are ellipsoidal to oblong in 

 shape, measuring about 4 X 8 fi. These spores were germinated in 

 Van Tieghem cells and the growth of the mycelium followed until the 

 formation of new bulbils, which were transferred to nutrient agar 

 media, where they produced mycelia and bulbils like the original 

 culture. 



On tube cultures this fungus occasionally produces typical sclerotia, 

 wlii.l i are formed by the massing together of many hyphal branches 

 which remain colorless for some time and thus are easily distinguished 

 from the bulbils. Moreover, they are larger, 400-500 fx in diameter, 

 irregular in shape, somewhat darker in color at maturity, and com- 

 posed of smaller, compact cells. 



Grandinia also produces conidia of the Oidium-type on slender 

 clampless conidiophores, such as are described by Lyman ('07) for 

 Cortieium alutaceum. 



CoRTICUM A.LUTACEUM (Schrader) Presailola. 



The bulbils of this species were obtained from Dr. Farlow, who found 

 them on a piece of rotten oak bark collected at Chocorua, N. H. It 

 was comparatively easy to get a pure culture, as the bulbils are pro- 

 duced in large numbers and germinate readily. This form has been 

 carefully compared with specimens of Cortieium alutaci urn obtained 

 from Dr. Lyman and they proved to be the same. The development 

 of the bulbil and the character of the conidia are practically identical 

 with those described for Grandinia and, as these have been well worked 

 out in pure cultures by Lyman ('07), it is not necessary to repeat the 

 results here, a detailed description of which may be obtained by con- 

 sulting his article, pp. 160 and 196. The mode of development of the 

 bulbils and the character of the conidia, however, have been carefully 



