HOTSON.— CULTURE STUDIES OF II NGI. 267 



and never, so far as the writer has observed, have any indications 

 been seen of the development of "spore balls," or perithelia such as 

 have been described by Biffen. 



BASIDlOMYi IETOUS FORMS. 



As has already been mentioned (p. 238), bulbils were first reported 

 among the Basidiomycetes by Lyman ('07), who not only definitely 

 connected one form with CoHicium alutaceum (Schrader) Bresadola, 

 which is dealt with briefly below, but also refers to two other kinds of 

 bulbils, the mycelia of which have well marked clamp-connections; 

 but basidiosporic fructifications were not produced abundantly 

 enough to allow of their identification. Dr. Lyman has kindly 

 supplied the writer with specimens of these forms for the purpose of 

 comparison, which will he referred to under their respective species. 



The methods used here were much the same as those already de- 

 scribed, except that more gross cultures of wood were used with 

 different amounts of moisture. The best results were obtained from 

 decoctions of bran in one or two litre Erlenmeyer flasks with pieces of 

 rotten wood that extended considerably above the liquid, so that the 

 mycelium could obtain the degree of moisture that best suited it. 



In order to keep the pieces of wood in place and thus lessen the 

 chances of contamination a quantity of agar was sometimes put in 

 the bottom of the flasks. 



Grandinia crustosa (Pers.) Fr. 

 Plate 6, Figures 1-10. 



Bulbils of this species were obtained from at leasl ten different 

 sources, mostly on substrata such as rotten chips of Live Oak {Quercus 

 agrifolia Nee), old canvas, paper, cardboard, etc., from Claremont, 

 California. It has been found also by Dr. Thaxter on gross cultures 

 of rabbit dung from Mass. and on rotten wood from Buenos Ayres, 

 and is probably the same as that referred to by Lyman ('07, p. L66), 

 which was obtained by Mr. A. If. Chivers on a gross culture of bits 

 of wood, paper, etc. 



Tin- mycelium, which shows quite marked clamp-connections, 

 is colorless, procumbent, producing numerous white fibrous, rope- 

 like strands of hyphae which radiate conspicuously in all directions 

 from the point of inoculation. The while mycelium, however, soon 

 takes on a light straw-colored aspect, owing to the formation of bul- 



