24 



KANSAS UNIVERSITY SCIENCE BULLETIN. 



two months old were inoculated in both acid and akaline 

 wort gelatin in Miquel flasks, and an additional alkaline wort 

 gelatin series was made in Petri dishes. Both flasks and 

 plates were placed in the dark at room temperature. The 

 results are found in the table given below : 



Table III. 



From this single experiment little can be deduced except 

 that both types liquefy wort gelatin in nearly the same de- 

 gree, any difference being in favor of the parent type, and 

 that liquefaction proceeded more rapidly in acid than in 

 alkaline wort gelatin. 



The most of the experiments described above were made 

 with a race originated about November 1, 1903. For the 

 sake of confirmation several other new races similar to this 

 one have been isolated from similar varying cells. I have at 

 present in my laboratory four such races of Saccharomyces 

 anomalus, ranging from one to three years and five months in 

 age, all of which came from vegetative "sports," and one 

 new race about two years old, arising from a spore. All of 

 these retain their new characteristics apparently undimin- 

 ished. Checks grown under parallel conditions clearly show 

 that the persistence of the new characteristics is in no wise 

 dependent on the medium or on other conditions of growth. 



The results of my work on Saccharomyces anomalus may be 

 summed up as follows : 



1. Continued selection of cells of more than average size 

 does not permanently modify the type. 



2. Variations occur in this species, which, like mutations 

 in higher plants, are capable of giving rise to races endowed 



