PLATE II. 



Saccharomyces anomalus grown in liquid media. 

 Figure 1. —The parent type from an old culture in wort. 

 Figure 2. —A new race of the same age grown in the same medium. 

 Figure 3.— A young culture of a new race grown in broth. 



Plates I, II and III are magnified 1000 diameters; plate IV, 250 diameters. 

 The yeasts represented in plate I were photographed unstained. Those in 

 plate II were stained in an aqueous solution of neutral red, and the bacteria 

 in plate III were stained with carbol fuchsin. Owing to shrinkage in stain- 

 ing, what were continuous filaments in the living culture of the bacteria 

 often show as chains of disconnected elements in the photomicrograph (fig. 

 2, plate III). In all cases the new races and the corresponding parent races 

 were stained alike and photographed with the same magnification. None 

 of the photographs have been retouched in any way. 



