Fig. 16. — Accessory and nucleolus shown. But one of the latter present. 

 It takes the safranin stain like the accessory. Every cell in this cyst 

 has but one of these nucleoli. Sometimes they show a tendency to lose 

 the safranin color and appear dark purple. Accessory is larger than 

 the nucleolus and happens in this case to lie against that part of the 

 nuclear wall which is uppermost; but nevertheless it is in contact with 

 the wall. 



Fig. 17. — From the same cyst as above. Accessory is vacuolated, as is 

 quite frequently the case. Nucleolus and accessory are at almost op- 

 posite sides of the nucleus. 



Fig. 18. — From a neighboring cyst but at same stage as figures 16 and 

 17. Shows condition of ordinary chromatin in figures 16-19. Nu- 

 cleolus and accessory lie close together but are separate. Nucleolus 

 is homogeneous but not nearly so dense as accessory, t, A tetrad be- 

 ginning to show the splitting of the thread, the longitudinal division. 

 p, A plasmasome, not found in every cell. 



Fig. 19. — From same cyst as figure 18. t, Possibly the end of a tetrad; 

 stained with safranin. It seems not to be typical for all the cells of the 

 cyst, for no others were found in the cyst showing this second body 

 so prominent or so deeply stained. 



Fig. 20. — Shows extreme looseness of the accessory. It is spread out 

 upon the inner side of the upper part of the nuclear membrane. The 

 nucleolus, besides becoming veiy irregular, is losing its staining power. 



Figs. 21, 22. — Slightly later stage than any of the preceding. The nu- 

 cleoli are stained purple instead of red. Many others of this cyst 

 have lost their stain. Some, however, still remain red. The nucleoli 

 appear to be still homogeneous. The small dark bodies present are 

 possibly small globules of chromatin already condensed. One acces- 

 soiy is vacuolated. 



Fig. 23. — Accessory shows indications of forming a spireme. 



Fig. 24. — Accessory is farther advanced. The nucleolus has lost its 

 safranin-staining ability, t, A tetrad that is in advance of its fellows. 

 The proximal ends are much condensed and stain safranin. 



Fig. 25. — Shows the spireme condition of four other accessories in the 

 same cyst from which figure 24 was taken. No indication of a special 

 bending in the middle. It is merely an irregular coil. The diameter is 

 very small and the length correspondingly great. 



Fig. 26. — A later condition. The accessory is shortened and its diameter 

 correspondingly increased. Five tetrads shown. XX, Parts of the 

 tetrad to which the fibers from opposite poles of the spindle will be 

 attached. Nucleolus entirely yellow or colorless but homogeneous. A 

 count of the chromosomes at this stage shows the full number twelve, 

 plus the nucleolus. It is therefore safe to say that the nucleolus is not 

 a chromosome. Another evidence of its nucleolar origin is the fact 

 that it degenerates and loses its staining ability. This takes place 

 about the time that the chromatin is being contracted into the form 

 of chromosomes. Clear spaces appear in the nucleus between the chro- 

 mosomes at this time. The nuclear wall is still intact. In general the 

 follicle is lightly stained as compared with other follicles and the 

 nucleoli are rather inconspicuous in the cells at all stages. 



