126 KANSAS UNIVERSITY SCIENCE BULLETIN. 



were placed in the bottle, and samples drawn from this bottle 

 were called the average for that day. Analyses for the week 

 were made on the following Saturday. 



In the chemical analysis the usual methods were adopted,' 

 with one variation, that being in the determination of nitrogen 

 as nitrates. 



Total Solids. — Fifty c. c. of the sample were evaporated on 

 the water-bath to dryness ; allowed to remain for twenty min- 

 utes, in order to have it as dry as possible, at the temperature 

 of the water-bath ; the bottom of the dish wiped with a clean 

 cloth ; and the dish was allowed to cool in a desiccator for one-half 

 of an hour, then weighed. Afterward the dish was heated to a 

 low redness and weighed, to determine the loss on ignition. 



Hardness. — This determination was made by diluting 25 c' c. 

 with 100 c. c. of pure distilled water and using the common 

 Clark method. 



Chlorine. — Fifty c. c. were titrated with a- standard silver- 

 nitrate solution, using potassium chromate as indicator. 



Oxygen Consumed, — One hundred c. c. were treated by the 

 Kubel method. 



Free Ammonia. — Five c. c. of the water were added to 500 c. c. 

 of ammonia-free water, and placed in a distilling flask, from 

 which 150 c. c. were distilled and nesslerized in the usual man- 

 ner. 



Albuminoid Ammonia. — To the water remaining in the flask 

 after the free ammonia had been determined were added 50 

 c. c. of alkaline permanganate solution, and the distillation and 

 nesslerization carried on in the usual way. 



Nitrogen as Nitrites. — Five c. c. of the water were diluted 

 to 50 c. c, acidified with one drop of hydrochloric acid, and 1 c. c. 

 each of sulfanilic acid and napthlamine hydrochloride added. 



Nitrogen as Nitrates. — Fifty c. c. were evaporated to dryness 

 in order to drive off the ammonia, diluted up to its original 

 volume, and placed in a Nessler jar; to this were added four 

 inches of aluminum wire and 2 c. c. of pure concentrated sodium 

 hydroxide ; then allowed to stand for twenty-four hours. One 

 c. c. of this solution was then diluted to 50 c. c. with ammonia- 

 free water, and nesslerized in the usual manner, to determine 



