PRATT: CYCLOLOMA ATRIPLICIFOLIUM. Ill 



There are more of such globules in the four cells of the terminal 

 portion (figs. 17, n', and 78, u') of the glandular hairs than in 

 the basal portion (figs. 17, w', and 78, u'). In the terminal cell, 

 and the cell just beneath, in certain of the glandular hairs are a 

 few very large oil globules (fig. 78, d") some of which have a vol- 

 ume thirty-six times as great as the other oil globules. The usual 

 tests do not indicate the presence of saponifying oil, volatile oil 

 nor resin in these globules. The cell contents of the terminal por- 

 tion of the glandular hairs appear yellow in formalin material. 

 In iodine solution, potassium iodide-iodine or chloroiodide of zinc 

 a portion of these cell contents stain dark blue. The stain with 

 iodine solution thus indicates the presence of starch. 



The cell contents of the terminal portion of the glandular hairs 

 did not dissolve out in 5 per cent potassium hydroxide in twenty- 

 four hours or one week. After remaining twenty-four hours in 

 concentrated potassium hydroxide solution all the oil globules 

 and most of the cell contents had been dissolved out. Portions of 

 the cell contents dissolved out in 10 per cent nitric acid and 10 

 per cent sulphuric acid. In concentrated sulphuric acid the cell 

 contents are stained orange, and a part of the cell contents are 

 dissolved out after 15 minutes. A part of the cell contents are 

 dissolved out in 15 minutes in sections treated with concentrated 

 nitric acid, but the cell contents do not change in color in this 

 strength of the acid. Apparently only the oil globules are removed 

 from hairs remaining in xylene, chloroform or ether for twenty- 

 fouj' hours or one week. The cell contents stain with safranin, and 

 portions also stain with hsematoxylin. 



Some reducing sugar was present in the cells of the basal por- 

 tions of both the clothing and glandular hairs. 



The presence of glucosides, mucilage, tannin, protein, calcium 

 oxalate or alkaloids was not demonstrated in either of the two 

 types of hairs. 



Botanical Laboratory, 

 University of Kansas. 



BIBLIOGRAPHY. 



Bentham, G., and Hooker, J. D. ('80). Genera Plantarum, Vol. Ill, 

 pp. 46-50. 



Britton, N. L. and Brown, A., ('13). Illustrated Flora of the United 

 States and Canada, Vol. II, p. 16. 



Coulter, John M., and Nelson, A. ('09). New Manual of Rocky Moun- 

 tain Botany, p. 164. 



Engler, a., and Prantl, K. ('89). Natiirliche Pflanzenfamilien, T. Ill, 

 Abt. la., pp. 58-62. 



