hunter: experiments with sand fly. 317 



tory. On November 7 the male monkey used to receive inocu- 

 lation became suddenly ill, growing- flaccid and motionless, save 

 for a high rate of i-espiration. He was later placed in charge 

 of Doctor Boughten, pathologist, and Doctor Skoog, neurol- 

 ogist, of the University. He was chloroformed, autopsied, and 

 the brain and spinal cord were studied by Doctor Skoog. This 

 monkey was taken from the University hospital group. It 

 now appears that some eighteen months before this monkey 

 was exposed to poliomyelitis. While the time is remote, never- 

 theless the exposure obviously introduces a complication which 

 will make it unsafe to depend on this instance, unless sub- 

 stantiated by many pure culture corroborations. 



This closed the work for the year 1911, and as far as our 

 knowledge goes is the first recorded instance of an endeavor 

 to apply the Sambon theory in this manner. 



The following year, 1912, the same method of work was con- 

 ducted, and to avoid any possibility of contamination six 

 monkeys were imported direct from India for this work. 



The season previous the flies were very abundant. The sea- 

 son of 1912 they were notably rare, due principally to the 

 flooded conditions of the creeks in the northern part of the 

 state and improper amount of moisture in the southern part 

 of the state. The almost total absence, therefore, of flies in 

 July, as well as a want of pellagrins in the proper stage, pre- 

 vented any successful inoculations at that time. 



P^rom the fall brood, however, inoculations were made with- 

 out waiting for the flies to bite the monkeys, but securing in- 

 oculations through maceration attended by the proper aseptic 

 precautions. The last inoculation was made on a female 

 monkey on December 16, 17, 18, 1912, using flies that had 

 emerged on the 12th and bitten the pellagrin on the 14th. 



Work for 1913 was conducted under a special fund fur- 

 nished by the Board of Educational Administration. 



Special attention has been given this season to the biting 

 habits of Simulium in nature. Hei*etofore we have experi- 

 enced little difficulty in encouraging the sand fly to bite the 

 patient, but no extended study had been given to the biting 

 habits in nature. 



During 1912 it was found that they would bite during the 

 mornings and evenings, sometimes entering the tent. The sea- 

 son of 1913, however, was not favorable in Kansas for the 

 development of the sand fly during the summer. 



