THE KANSAS UNIVERSITY 
SCIENCE BULLETIN. 
VOLaVilaNow al JANUARY, 1912. Wows seus 
CYTOLOGICAL STUDIES OF FEMUR-RUBRUM AND 
OTHER MELANOPLI. 
BY NADINE NOWLIN. 
(Plates 28-32.) 
(Contribution from the Zodlogical Laboratory, No. 200.) 
IF TEEN years ago Wilcox (’95) published a paper on the 
KF spermatogenesis of Melanoplus femur-rubrum, or Calop- 
terus femur-rubrum, as the genus was then called. It is 
generally.conceded that many of his interpretations are incor- 
rect, but up to the present time his work stands as the only 
description of this species. Since it is my purpose to examine 
the chromosome complexes of as many genera of Melanoplus 
as possible, it seemed advisable to give emphasis to the dis- 
cussion of this particular one. 
Of the some dozen species that I have examined, femu2'- 
rubrum is perhaps the least favorable for study. The stage in 
which the chromosomes separate well are very short, and 
counts are difficult. This may explain some of the errors that 
Wilcox made. 
MATERIAL AND METHODS. 
The grasshoppers were collected in September, 1910, on the 
south campus of the University of Kansas. This is not the 
best time for collecting, as the germ cells are nearing the end 
of their activity, but all stages can be found from spermato- 
gonia to spermatozoa. Wilcox’s material was collected also in 
the fall. I have used Flemming’s and Bouin’s fluids for pre- 
serving, and both gave good results. Heidenhain’s iron-hema- 
toxylin was the chief stain, but Flemming’s tricolor was also 
tried. 
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