THE KANSAS UNIVERSITY 

 SCIENCE BULLETIN. 



Vol. IX, No. 11. | DECEMBER, 1914. [Z"xi£™*h 



Numerical Relation of Spermatozoa to 



Sertoli Cells. 1 



FLORENCE S. HAGUE. 

 [Contribution from the Zoological Laboratory No. 213.] 



THIS study was started with the purpose of ascertaining 

 the number of spermatozoa associated with each Sertoli 

 cell in the human testis. However, on account of being unable 

 to get material which was in the right condition, the problem 

 was changed to lower mammals. - 



The tissues used were from the testes of man, the cat, and 

 the rat. Nothing was known as to the age or condition, 

 healthy or otherwise, of the human material. The pieces sec- 

 tioned had been pieserved in formalin. Several celloidin 

 mounts were also stud'ed. Flemming's tri-color, Davis' cyto- 

 logical stain, and iron hematoxylin with acid fuchsin, and 

 eosin, as counter stains, gave the best results. Delafield's 

 hsematoxlyn with orange G, congoroth, and bordeaux red re- 

 sulted in very lightly stained sections. For fixing the rat testes 

 Mueller's, Gilson's, Bouin's, Tellyesniczky's and Zenker's fluids 

 were used, the last two of which were the most satisfactory. 

 The cat testes were preserved in Tellyesniczky's and Flem- 

 ming's solutions. For sections of both the rat and cat testes 

 tri-color, iron hematoxylin with or without eosin, dilute Dela- 

 field's hematoxylin and hsemalum were used. The sections 

 varied in thickness from 6 to 40 micra. 



1. I wish to acknowledge the assistance and direction of Prof. W. J. Baumgartner 

 and Dr. B. M. Allen in this problem. 



2. The numerical relation is now being worked out in the eat. A similar study is 

 planned for other forms. 



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 2 — Univ. Sci. Bull., Vol. IX, No, U. 



