288 KANSAS UNIVERSITY SCIENCE BULLETIN. 



Pieces of the bark were boiled in water. Some of the water 

 boiled with Fehling's solution showed minute crystals of 

 cuprous oxide. These were removed by filtering, more Feh- 

 ling's solution was added, and the solution was left in an elec- 

 tric oven over night. No more crystals formed, showing that 

 no glucoside soluble in hot water had been formed. A section 

 boiled in Millon's reagent showed the cell contents red in some 

 of the cells of the medullary rays, some in the phloem, and 

 many in the parenchyma of the bark. The cambium was a 

 continuous line of red. Millon's reagent caused a heavy white 

 precipitate in the parenchyma of the bark. Sections of 

 the bark soaked in dilute potassium hydroxide, chloro- 

 form, alcohol, hot water, and salt water still gave the pre- 

 cipitate on being treated with Millon's reagent. A section 

 soaked in dilute HC1 and then treated with Millon's reagent 

 gave no precipitate showing that salts either of calcium oxalate 

 or calcium carbonate are present in the bark. A section was 

 treated with HC1, and since no bubbles of gas were given off 

 the precipitate must be due to calcium oxalate and not to 

 calcium carbonate. 



A section treated with nitric acid and then with ammonia 

 showed the presence of much proteid in the bark beginning 

 with the cambium. Ferric chloride, copper acetate, and 

 chromic acid all showed the presence of tannin in some cells 

 of the phloem and in most of the parenchyma of the bark. 

 Bits of bark were put into dishes containing alcohol, alcohol 

 ether, and xylene. The next day bits of Swedish filter paper 

 were put into the dishes and the fluid allowed to evaporate. 

 Then part of the pieces of filter paper from each dish were put 

 into alcannin and part into Sudan III, testing for oil, and 

 part into copper acetate as a test for resin, with the following 

 results : 



I. Alcohol extract. 



a. In alcannin. Small red bodies in great numbers clinging to 



the fibers of the paper. Also many larger bodies composed 

 of aggregations of the smaller ones. 



b. In Sudan III. Same sort of bodies stained bright red. 



c. In copper acetate. Same bodies stained a deep brown. Fibers 



of the filter paper also covered with a brownish-green film. 



