BAIRD: ANATOMY OF PLAT ANUS. 289 



II. Alcohol-ether extract. 



a. In alcannin. Very numerous large bodies with irregular out- 



line stained red. 



b. In Sudan III. Same bodies bright red. 



c. In copper acetate. Same bodies brown. 

 III. Xylene extract. 



a. Alcannin. Red patches in great numbers without definite out- 



line. 



b. Sudan III. Same spots or patches stained a yellowish-red. 



c. Copper acetate. Fibers of the paper were covered with a 



grayish-green film. Denser patches between the fibers were 

 of the same color. 



Since the material extracted had every appearance of being 

 oil when treated with alcannin and Sudan III, and since it did 

 not become emerald green when treated with copper acetate, 

 as resin would do, the conclusion would be that it was oil, but 

 on being treated with Tunmann's saponifying reagent there 

 was no saponification. 



Wood. 



The medullary rays and pith of young wood and the rays 

 of older wood are loaded with starch of different kinds, as 

 shown by the different shades of blue when stained with 

 iodine. There is also starch in the wood parenchyma. 



Fehling's solution showed that sugar is associated with 

 starch in the pith, wood parenchyma and medullary rays. 



Alcannin and Sudan III showed no oil in the wood. 



Bits of wood were put into alcohol, alcohol-ether, and xylene, 

 and the same tests were made as the last recorded above for 

 the bark. Nothing was extracted by these solvents, showing 

 that the wood does not contain oil or resin. 



Methylene blue did not show mucilage. Glucoside tests 

 showed the presence of no glucosides in the woocf. 



Millon's reagent did not give the reaction for proteid in a 

 section of wood five years old. In young wood it showed 

 occasional cells in the pith and medullary rays containing 

 proteid. 



A section of wood treated with nitric acid and ammonia on 

 a slide showed occasional cells in pith and rays which contain 

 proteid, such cells being stained a deep yellow. 



Ferric chloride showed tannin in the wood parenchyma, and 

 in some of the cells of the medullary rays and pith. 



Botanical Laboratory, 

 University of Kansas. 



