TUCCA ANP.USTIFOI.IA. 2G9 



Squibb's stronger alcohol was used. A dark red-colored liquid was extracted. It 

 was neutral in reaction with litmus. The alcoJiolic extract was evaporated in a current 

 of carbonic acid. The residue was non-ci-ystalline and of a I'cd color. A definite volume 

 of the alcoholic extract was evaporated, dried until the weight remained constant, and 

 the residue incinerated in a weighed platinum dish and the ash estimated. 



I. 



TOTAL SOLIDS. 



Akoliolk residue dried at lOOO C 9.25 per cent. 



" " " lioo C 0.25 



" fisli 0.2 



The alcoJiolic residue was treated with distilled water, and a definite volume of 

 the extract was evaporated, dried, and weighed. The alcoholic residue insoluble in 

 water was treated with water containing ammonia (one part in fifty). This ammoni- 

 acal extract was evaporated with excess of acetic acid, and the residue rinsed with a 

 little water on a filter, dried, and weighed. The dried aqueous extract insoluble in 



ammonia was then estimated. 



II. 



Distilled water residue ;'.'-'2 per cent. 



Ammonia" " •">.-t3 " 



Insoluble '• " O.fiO " 



Total solids 9.25 



The aqueous extract from the alcoholic residue was studied as follows : It was 

 not colored by a ferroso-ferric salt nor precipitated by gelatine and alum solutions, 

 showing absence of gallic acid and tannin. A portion of the aqueous extract was 

 acidified with sulphuric acid and agitated successively with petroleum spirit, benzole, 

 chloroform, and amyl alcohol. The acidified liquid was rendered alkaline by ammonia 

 and agitated with the solvents in the same order. Petroleum spirit removed from the 

 acidified solution traces of an amorphous residue, soluble in sulphuric acid and caustic 

 soda. Benzole and chloroform separated no substances from the solution. As the 

 amyl alcohol solution was evaporating white needle-shaped crystals were seen fioating 

 in the liquid. On drying the residue they were decomposed and melted, leaving a dai k- 

 colored liquid. Several attempts were made to dry these crystals, without success. 

 A few of the crystals were recovered from the solution, and tested for alkaloids; no 

 reactions were obtained with the usual reagents for them. 



Glucose was estimated from the aqueous extract. The licpiid was heated over a 

 water bath with Fehling's solution, and the pi-ecii)itated red cui)rous o.vide was thrown 

 upon a weighed filter, dried, and incinerated. The glucose was estimated gravimetri- 



