TUCCA ANGUSTIFOLIA. 277 



Carhhy (Irate Filtrate. 



The carhlnjdrate filtrate was concentrated at a low temperature in a ciu-rent of 

 carbonic acid until the alcohol was dissipated. The residue was examined for glucose, 

 organic acids, saponin, and tannin. Traces of glucose were detected qualitatively by 

 Fehling's test; the amount of cui^rous oxide present was too small to estimate gravi- 

 metrically. A i)ai-t of the carhhydrate filtrate residue was boiled with 83 per cent, 

 alcohol, and filtered Avhilc hot. On cooling, a precipitate formed. This precipitate was 

 identified as saponin. It was almost insoluble in stronger alcohol. Baryta-water pre- 

 cipitated it from aqx;eous solution. Its aqueous solutions frothed on shaking. When 

 agitated with chloroform and on evaporating the chlorofonn solution, a light-colored 

 residue was obtained. A few drops of concentrated sulj^huric acid mixed with it gave 

 a reddish-A-iolet color reaction. Another portion of the carhhydrate -filtrate residue was 

 precipitated with neutral acetate of lead and filtered. The precipitate was suspended 

 in water, decomposed by sulphuretted hydrogen, and the lead sulphide filtrate evapo- 

 rated over a water-bath to expel all odor of sulphuretted hydrogen. The liquid was 

 cooled and lime-water added until the reaction was alkaline to litmus. A turbidity 

 forinc<l when the lime-water was added to the filtrate, and was not entirely cleared 

 on the addition of dilute acetic acid. A neutralized portion of the lead sulphide 

 filtrate gave a yellow precipitate with a ferrous salt. Oxalic acid by these tests was 

 indicated, and possibly other vegetable acids were present in the filtrate. The carh- 

 hydrate filtrate residue was examined for tannin, and with a negative result. Calcium 

 oxalate was separated. 



Extract (2), the Wood of the Root. 



The powder used in the alcoholic maceration was thoroughly dried, and replaced 

 In tlir poicolator. A measured quantity of cold distilled water was allowed to percolate 

 slowly tlirough the powder. The extract was colored, and slightly acid in reaction. A. 

 definite volume of the extract was evaporated, dried, and weighed. A known weight 

 of the residue was incinerated in a weighed covered porcelain crucil)le, and the ash 

 determined. The ash was white and incompletely soluble in water. 



TOTAI, SOLIDS. 



Aqueoui residue dried between lOO^ and llO'^ (' 12.10 per cent. 



" ash 1.74 



Oum. 



A certain quantity of the a/piexnis extract was mixed with two volumes of stronger 

 alcohol (Squibb's). The mixture was allowc'l to stand ini- twenty-fnui- hours, and the 



