1917] Mitra: Toxic and Antagonistic Effects of Salts on Wine Yeast 97 



Relative Antagonisms of Various Combinations 



Table 12 is intended to show the relative antagonisms of the 

 various combinations. The data used in constructing the table are 

 the final counts in each flask. 



The average of the counts in all the check flasks is taken as the 

 basis from which to estimate the influence of the various salts and of 

 their combinations. The calculation is made as follows : 



Yeast growth in check flasks=17 (millions) . 

 Yeast growth with single salt no. 1 = a. 

 Yeast growth with single salt no. 2 = o. 

 Yeast growth with combination no. 1 + 2 = c. 

 Toxicity — expected = (17 — a) + (17 — b). 

 Toxicity — observed = 17 — c. 



Antagonism of combinations* = (17 — a) + (17 — b) — ■ .(17 — c). 

 ' Antagonism = 17 + c - — a — b. 



Table 12 — Bange of Antagonism op the Binary Combinations Calculated 

 From the Last Microscopical Count* 



No. MgCI 2 X CaCl 2 KC1 X CaCl 2 MgCl 2 X NaCl KC1 X NaCl KCIX MgCl 2 CaCl 2 X NaCl 

 At 17,000,000 17,000,000 17,000,000 17,000,000 17,000,000 17,000,000 



B 



C 4,000,000 5,000,000 4,000,000 



D 4,000.000 17,000,000 8,000,000 10,000,000 4,000,000 



E 27,000,000 17,000,000 8,000,000 9,000,000 9,000,000 7,000.000 



F 24,000,000 18,000,000 25,000,000 8,000,000 7,000,000 7,000,000 



G 20,000,000 30,000,000 27,000,000 7,000,000 16,000,000 6,000,000 



H 21,000,000 31,000,000 14,000,000 7,000,000 9,000,000 



I 7,000,000 8,000,000 11,000,000 10,000,000 



J 1,000,000 1,000,000 2,000,000 10,000,000 



K 1,000,000 



L - 



M 



N 



O 



P 



f Millions on average. 



* These results are shown graphically by the curves in figure 12. 



* This defines 'antagonism' as the difference between the expected and the 

 observed toxicity. 



The curves have been drawn to show the antagonism of the com- 

 binations and not the actual growth of the yeast as has been shown 

 in the previous curves. 



