575 Douglas H. Campbell. 



that the spermalozoids swarmed in a dilute dahlia Solution and were more 

 or less deeply stained, but it was impossible to get a sufficient number to 

 make more thorough investigations. 



The arehegonium had the nuclei of the neck-cells somewhat colored 

 after which the neck opened in the usual manner. 



With the small protbaliia of the dioecious ferns undoubtedly the results 

 would be better, but these unfortunately were not obtainable when the ex- 

 periments were made. 



Lilium hulhiferum L. 



The bases of the perianth divisions of this lily, in common with some 

 others, is characterized by parallel ridges fringed with hairs, some of which 

 are unicellular, others mullicellular. In the species under consideration 

 most of these are colored by the presence of apurple-redcell-sap andyellow 

 chromoplasts, but a number can always be found that are colorless. The nu- 

 cleus is large and distinct and the Streaming good. As in the case of most 

 aörial hairs, however, the thick walls of the cells make the absorption of 

 coloring agents difficult so that results are rather uncertain. 



The colors employed were mauvein and dahlia and the Solutions ranged 

 from -001 ^ — -0002 %. With all the Solutions a fair number of cells were 

 found to have the nucleus colored, but it was necessary to leave them in the 

 Solutions for several hours. The best coloring was reached with a -001 % 

 Solution of mauvein in which the separated bases of the petals were allowed 

 to remain all night. In one case an extraordinarily deep violet-purple stain 

 was thus obtained with evident though much diminished Streaming. 



Asphodelus albus Nees. 



The hairs fringing the broad bases of the filaments of this plant were 

 examined with fairly good results, though owing to the thick cuticle they 

 are not always to be relied upon. They are of large size, unicellular, and 

 perfectiy colorless. As in the case of Lilium bulbiferum, a long Immersion is 

 necessary and the same Solutions may be used. With all of the Solutions cases 

 of stained nuclei were observed, but in no cases was the color particularly 

 deep. 



If too streng a Solution is used, — and the same is true of Lilium^ — 

 the cell-wall colors very intensely, so as to make it impossible to determine 

 positively whether the nucleus is colored or not. 



Sagittaria sagiUifolia L. 



The epidermis of the base of the leaf-stalks of this plant offers an easily 



prepared and satisfactory subject. The cells contain very little protoplasm 



and Chlorophyll, and the nucleus is large and distinct, Extremely fine proto- 



plasmic Ihreads traverse the cell-lumen and in these there is active Streaming. 



