1912] Gooclspeed: Nicotiana Hyhrids 135 



and an intermediate variety (17 to 24 mm.) This matter will 

 be further discussed in connection with the measurements of 

 corolla diameters of hybrids and parents in 1911. 



3. Cross Pollinations made between Parents 



Between September 2 and October 5 seventeen cross-pollina- 

 tions were, to all appearances, successfully performed. The cross 

 I5X II d^ was made twdce and its reciprocal II $X I d^ twice. 

 Variety I was crossed with variety III on two occasions and 

 IIIJX I c^ twice also. Cross II 5 X HI c^ was made five times 

 and III 5X II cJ* four times. To obtain pollen for these cross- 

 pollinations twenty-two sets of buds were prepared and bagged 

 and twenty-eight female parents were castrated and bagged. 

 In variety III there was a most annoying tendency of the buds 

 that were being worked with to snap off and it was quite diffi- 

 cult to perform the castrations successfully. Fully opened 

 flowers were not so delicate in this respect. Fourteen of these 

 crosses had been made before September 7 and on the 20th the 

 bags were opened and a few small buds, inconspicuous and 

 overlooked in preparing the lateral two weeks before, were picked 

 off. In all but three cases seed seemed to be forming. The 

 three doubtfully successful crosses were repeated. All seed was 

 gathered and hung to dry before October 15. 



Because of the extent to which variety III dropped its buds 

 this parent was artificialh' self- (close) pollinated. In the other 

 two varieties there was no difficulty in obtaining naturally self- 

 ( close) pollinated seed. The parental seed was gathered and 

 hung to dry along with the hybrid seed. 



