472 University of California Puhlications in Botany [^'or.. 



4. F3 AND SUBSEQUENT GENEEATIONS OF THE ANGUSTIFOLIA- 



MACROPHYLLA SERIES 



From F, of Ho and H„ 20 plants were selected for further ex- 

 perimentation and families of 25 were determined upon as the unit. 

 In all except four, the families of 25 each were successfully raised. 

 Of one of the four only 14 plants were obtained, which were all that 

 germinated, while in each of the other three families 24 plants were 

 reared to maturity. Altogether, then, 486 plants were raised of the 

 Fg during the season of 1912. It was the intention to grow from each 

 of the selected types, as drawn for illustration. Fifteen of the families 

 from the type parents were successfully reared, but, in some way or 

 other, the seed of type 4 (lOFoHoP^Pig) was not to be found, and, 

 unfortunately, no note had been made as to whether or not any seed 

 was produced. No complete sterility, however, Avas noticed in any 

 members of F, of either Ho or H^, and the presumption is that Fo seed 

 of type 4 must have been lost in harvesting. 



The variation within each family was decidedly less than that of 

 the families of Fo. Of the 20 families reared wholly or in part, 4 

 families were very nearly uniform, varying in minor details only. 

 Five families segregated only in corolla color, 4 segregated only in 

 leaf base characters, and the remaining 7 segregated both in corolla 

 color and leaf base characters. In table 2 are summarized the data 

 as to gro.ss behavior of these families of F3. In those families grown 

 in F^ and subsequent generations, a definite attempt was made to fix 

 the original characters of the Fo type selection in a pure line. The 

 genealogical relation of these selected lines to each other is shown in 

 the chart reproduced herewith. The letters. A, B, C, etc., correspond 

 to the Fo family designations noted in table 1, and the numbers refer 

 to type selection numbers corresponding to the type illustrations in 

 plate 63 to 78, or, in the case of types 17 to 21, corresponding to those 

 types as described in the succeeding accounts of the later generations. 



