260 



University of California Publications in Botany [Vol. 5 



Table 2 which follows gives the length and breadth of the seed of 

 a few of the plants mentioned in table 1. The averages are based 

 on approximately 20 measurements in each case. For two of the 

 castrations which produced some normally matured seed (plants 1-4 

 and 36. below) measurements are given for the length and breadth 

 of the normal seeds and also of the abortive seed. Many of the seeds 

 with ' ' empty seed-coats ' ' were of full size, the figures given represent- 

 ing average sizes. It was possible to select from the abortive seed of 

 one capsule over 75 seeds which were indistinguishable in size from 

 the seed resulting from self-fertilization. 



TABLE 2 



Plant 

 No. Date 



16 



Treatment 

 self-fertilized 



14 9/26 castrate(i(?) 



9/26 castrated (?) 



21 9/26<='> castrated 



36 9/] 8 castrated 

 9/18 castrated 



44 9/29<^> castrated 



Average Average 



length, breadth, 



mm. mm. 



0.39 



Remarks 



0.2.J Normal seeds that germinated over 

 80 per cent. 



0.37 0.24 Castration judged to be contami- 



nated (cf. p. 254) — seeds with 

 endosperm and embryos. 



0.26 0.20 Seeds of same doubtful castration 



— empty seed-coats only. 



0.26 0.19 Empty seed-coats only. 



0.38 0.25 Seeds with endosperm and embryos. 



0.27 0.21 Empty seed-coats only. 



0.27 0.19 Empty seed-cor.ts only. 



It is thus possible to state that of some 800 castrations and mutila- 

 tions divided approximately evenly between the 95 plants of ''Nic. 

 tahaccuni Cuba" grown this past year, there were 112 instances, 

 involving nearly 200 flowers, in which one or more fruits developed 

 to normal size and matured seed, almost all of which was normal in 

 appearance, though small in size, and a small proportion of which 

 was normal in every way including the presence of endosperm and 

 embryos. 



During the past ten years a very considerable collection of Nic- 

 otiana species and varieties has been grown in the pure line in the 

 Botanical Garden of the University of California. They have been 

 fully described elsewhere (Setchell, 1912). Every year two or three 

 plants of each of the species and varieties listed below have l^e^^n 



