CHYTRIDIALES 469 



formed, further strengthens the view that the two are identical. See also 

 remarks under the next species. 



Striking features of the species are its large oil globule in the 

 zoospore and strong tendency for extramatrical development. It re- 

 sembles in the latter respect a Nowakowskiella rather than a Clado- 

 chytrium. 



Cladochytrium granulatum (Karling), comb. nov. 



Nowakowskiella granulata Karling, Bull. Torrey Bot. Club, 71: 374, figs. 

 1-29. 1944. 



Rhizoidal system profusely developed, much branched, hyaline when 

 young, brownish and thick-walled with age, slender parts 1.5-7 \x in 

 diameter, expanded parts mostly nonseptate, oval (6-8 by 9-11 \jl), 

 broadly fusiform (5-9 by 8-13 \i), almost globose (6-10 \x in diameter), 

 or irregular; sporangia terminal or intercalary, mostly nonapophysate, 

 globose (12-35 \i in diameter), pyriform (12-22 by 15-30 jx), oval (10-18 

 by 12-25 [j.), or occasionally irregular, with one to three discharge 

 papillae, 3 by 5 ;jl, or one terminating a more or less elongate discharge 

 tube which at its tip bears a plug of opaque gelatinous material and 

 within it on the surface of the contents proper an oval, discoid, crateri- 

 form, saucer-, bowl-, cup-, or cone-shaped endooperculum, 3-7 [jl in 

 diameter; zoospores globose, 5-6.6 \x in diameter, with numerous 

 golden-brown granules of uniform size in the contents and a flagellum 

 about 35 \i in length; resting spores formed from the intercalary swell- 

 ings, with a smooth, hyaline 1.5-2 [i thick wall, globose (15-24 [i in 

 diameter), oval (15 by 20 \x), with a large (12 (x in diameter) refractive 

 globule and numerous smaller ones, germination not seen. 



In decaying vegetable debris, Karling (be. cit.), Brazil. 



As Karling recognized, the fungus strongly resembles a Cladochytrium. 

 Since the type of operculation is not regarded here as comparable to 

 that of a true species of Nowakowskiella, the species is placed in Clad- 

 ochytrium. It has been suggested that the formation of the poorly 

 defined endoopercula and the presence of Cladochytrium-like plugs of 

 gelatinous material in the orifices of the discharge tubes points to the 

 species as transitional between the inoperculate and operculate forms, 

 or vice versa. Such a possibility is worthy of consideration. The 



