CHYTRIDIALES 585 



containing one to many oil globules. Germination of resting bodies not 

 observed" (Whiffen, loc. cit.). 



Saprophytic on a grass leaf in soil, United States. 



? Nowakowskiella profusa Karling 

 Bull. Torrey Bot. Club, 68: 386, 1941 ; 71 : 382, figs. 45-68. 1944 



(Fig. 35 H, p. 583) 



Rhizomycelium very profuse, extensive, and richly branched, hyaline, 

 1-9 [i. rarely 6-15 \x in diameter, well-defined, septate, spindle-shaped 

 and oval swellings rare or lacking; rhizoids abundant; zoosporangia 

 terminal or intercalary, hyaline, smooth, rarely apophysate, spherical 

 (10-45 \x in diameter), ovoid, ellipsoid (8 by 12-20 by 35 \x), clavate, 

 obclavate (10-22 \x by 15-40 [x), pyriform, obpyriform, elongate, cylin- 

 drical (8 by 25-10 by 50 y.), lobed and irregular; operculum apical, 

 subapical or lateral, oval and spherical, 4.4 \x-l \x, remaining attached 

 to the sporangium or lying nearby; zoospores hyaline, spherical, 4 u,- 

 5.5 [x with a small refringent globule, 0.7-2 ;jl; resting spores terminal 

 or intercalary, spherical (14-25 \x), ovoid, ellipsoid (10 by 15-14 by 22 fx), 

 truncate, spindle-shaped, and rarely irregular, with a fairly thick, 

 yellowish brown, smooth wall, content granular with numerous refrac- 

 tive globules, germinating after a short rest period, forming a simple or 

 branched, tapering, thin-walled, operculate exit tube and producing 

 zoospores directly within, or functioning as a prosporangium and 

 forming a thin-walled zoosporangium on the surface. 



Saprophytic in decaying vegetable debris, Karling (1944b: 382), 

 rotting oat leaves, Karling (1941b: 108), Karling (1942c: 620), on cel- 

 lophane and onion skin, from soil, Karling ( 1 948c : 5 1 0), J. Roberts ( 1 948 : 

 147, fig. 2), United States; soil, Reinboldt (1951: 178), Germany. 



J. Roberts (1948) reported that the optimum temperature for growth 

 on solid cellulosic media of his isolate assigned to this species was 24- 

 28° C. He noted that in the same layer the walls showed a predominance 

 of chitin mixed with cellulose. 



? Nowakowskiella crassa Karling 

 Bull. Torrey Bot. Club, 76: 294, figs. 1-15. 1949 

 Rhizoidal system hyaline, profuse, branched, usually large and 



