642 AQUATIC PHYCOMYCETES 



In larvae of Anopheles gambiae (syn. A. costalis), Sierra Leone. 



Four types of resting spores were found by Walker (op. cit.) in mate- 

 rial obtained from various places in Sierra Leone. To the most common 

 (his "Type 3") he gave the name Coelomomyces africanus. Although 

 the others are without significant descriptions, it is evident from the 

 the photomicrographs that at least one of them (PI. 3, fig. 4 "Type 4") 

 is distinctive. It will no doubt prove to be new. The resting spores of 

 Walker's types 1 and 4 were 45-60 jjl long; those of his types 2 and 3 

 varied from 25-35 [x. 



Walker unsuccessfully attempted to infect larvae with undried resting 

 spores of Coelomomyces. As Couch ( 1 945b) explained, it is likely Walker's 

 negative results were due to the fact that he was dealing with a species 

 requiring drying of the resting spores before germination. 



Coelomomyces dodgei Couch 



J. Elisha Mitchell Sci. Soc, 61: 129, pi. 1. figs. 1-4. 1945. Emend. Couch, 

 in Couch and Dodge J. Elisha Mitchell Sci. Soc, 63: 71, pi. 16, fig. 1, pi. 20. 



fig. 1. 1947 



Hyphae 7-14 u. thick irregularly branched and anastomosing; spo- 

 rangia 27-42 by 37-65 [j., oval, sometimes slightly flattened on one side, 

 wall deep brown, rarely hyaline, 1.5-4.2 \x thick, consisting of an outer 

 thickened hyaline to brown part (2-3 jjl thick) and an inner thin hyaline 

 layer (1-2 \x thick); outer part of wall with rounded or elongated pits 

 or with narrow bands separated by grooves ; not infrequently with narrow 

 bands on one side and pits on the other; the grooves extending length- 

 wise or arranged in variously curved patterns; bands about 3.6 u. wide 

 and not very distinct, with 7-10 visible on surface view; wall with a 

 preformed longitudinal groove along which dehiscence occurs to permit 

 spore discharge; germinating in water and without previous drying 

 after the death and partial disintegration of the larva, the process 

 occupying 24 to 36 hours; when ready to germinate, the contents swell- 

 ing, producing a lateral bulge and thus causing the thick outer wall to 

 crack open at the longitudinal groove; twenty-four to thirty-six hours 

 later, the spores emerging through this crack, surrounded by a gelatinous 

 material which alter three to five minutes disappears, allowing the 

 spores to escape; spores posteriorly uniflagellate, elongated and broad- 



