ENTOMOPHTHORALES {ANCYLISTES) 1069 



Ancylistes closterii appears to be the commonest species of the genus, 

 although, as Berdan suggests, some of the records based on sterile 

 material may actually refer to A. berdanii ("A.pfeifferi"). Pfltzer's spe- 

 cies is moderately frequent in bogs in Northern Michigan. 



Berdan noted a profuse production of external hyphae in Ancylistes 

 closterii; she counted as many as 65 radiating to a distance of 1.2 mm. 

 from a single host cell. 



Ancylistes berdanii Sparrow 

 Trans. Brit. Mycol. Soc, 38: 217. 1955 

 (Fig. 91 G, p. 1066) 

 Ancylistes pfeifferi Beck, sensu Berdan (1938) 



"Intramatrical mycelium irregular, 7.5-14 fx, usually 9.5-1 1 \x, before 

 septation; enlarged at the free ends and at intervals along the hyphae; 

 segments or cells distinctly femur-shaped, 10.5-11.5 jx in the center, 

 1 3-19 u. at the broad ends, 9.5-12.5[x, usually 10.5-12.5 jx at the septa, and 

 43-67 u, long; segments with terminal vacuoles and several intermediate 

 ones, and highly granular cytoplasm. Segments of external hyphae 4-7 tx 

 X 44. 5-1 04 fx, average 45-70 ;x long. Conidia hyaline, spherical, 21-23. 5;x 

 in air, 22.2-30 [x with papilla in water, germ tube 4.5-5 [x; appressorium 

 at end of infection cell disc-like, 3-4 [x, later appearing as a flat plate 

 with a hyaline, gelatinouslike sheath which extends backward to broad- 

 er part of infection cell; infection cell 7.5-13 fx x 37-67 [x, usually 1 1- 

 12 [x x 40-55 u,; penultimate cell often very short and broad, 6-10 fx x 

 9-26 [x; entrance tube penetrating appressorial plate as a fine papilla. 

 Conjugation lateral or scalariform ; gametangia cut off from vegetative 

 cells by 1 or 2 septa depending upon their terminal or median position; 

 male gametangium straight, narrow, sometimes with several blunt 

 branches in cases of lateral conjugation; female gametangium swollen; 

 cell enclosing zygote 30-40 [x, somewhat stellate with the wall extending 

 out at intervals in 9-10 broad, bluntly truncated protuberances and 

 separated from the main filament by a short stalk cell. Zygote or resting 

 spore spherical, 18.5-22 [x, smooth, brown, thick-walled and filled with 

 refractive globules; retracted from wall of enclosing cell and lying free; 

 germination unknown" (Berdan, 1938). 



