318 



BIOCHEMICAL SYSTEMATICS 



:ii-w^ ' 



Fig. 15-2. (a) Baptisia leucophaea; (b) B. sphaerocarpa; (c) B. nuttalliana; (d) ultra-violet 

 photograph of leaf extract from a hybrid between B. nuttalliana and B. leucantha chro- 

 matographed in two-dimensions. 



lengthy study of numerous individuals of the three species concerned, 

 methods of two-dimensional chromatography were developed which 

 allowed the detection (in ultra-violet light and after spraying with a 

 general phenol-detecting reagent) of numerous compounds from leaf 

 extracts. These compounds fell into one of four classes: 



(1) substances common to two or even all three species 



(2) substances which are species-specific and highly reliable 

 because of their distinctiveness and consistent presence 



(3) species-specific substances not constant for the species 



