30 Botanical Microtechnique 



the container. Pour the fluid, tor instance once-usecl anhydrous butyl 

 alcohol, into the jar. As the alcohol accumulates, shake the jar 

 occasionalh. Allow to stand for a day and decant the liquid onto 

 anhydrous calcium suljihate, known commercially as "Drierite." After 

 several hours, decant and filter the fluid and it is ready for use. If 

 the fluid has become colored from materials extracted from the tis- 

 sues, distill at the boiling point of the reagent being distilled. This 

 information can be found in a chemical handbook. 



Wet calcium chloride can be regenerated by drying first in dry 

 air, then in an oven at 110°C. Drierite can be regenerated by air 

 drying and then heating in a furnace at 225 to 250°C. for two hours. 



