52 Botanical Microtechnique 



the concave ends ^vith a pair of needles. Allow the ribbon lo cool, 

 then blot with lintless filter paper. Wipe excess adhesive from around 

 the edges of the ribbon, otherwise a ring of stainable adhesi\e may be 

 left on the slide and disfigure the finished preparation. 



After the sections have been warmed and blotted and the excess 

 adhesive wiped off, put the slides into the 53° oven or some other 

 Avarm place free from dust. The adhesive becomes hardened enough 

 in about 4 hr. to hold soft materials such as root tips. For hard 



Bryophyilum 

 leaf 



B 



F F 



Fi(.. (). 6— Spacing and anaiiocnient of sections (ribbon) in relation H) si/e and 



character of snbject and size of available cover glass. 



materials a 12-hi. interxal is necessary. After the necessary inter\al, 

 remove the slides, and store in slide boxes in a dust-free place. 



Numbering and Recording of Slides 



Jt is iiecc'ssaiN to number or otherwise inaik research oi demon- 

 stration slides afler affixing the jKuadin rii)i)()n. llu' seiial iHniiI)ei 

 or (odf kllei of the main i;il (an l)i' scratched on llu slide willi a 

 diamond, carbide steel oi ( arboi inidtmi j)encil. Waicipiool India 

 ink diluted with an e(|iial \()liiine ol gelatin adiiesixe iiiaki's an 

 excellent slide maiking ink that will adhere to (lean glass through 

 the entire staining process. 



In some in\ esiigations it is necessary to make a comjileie series 

 of sections fiom a pie(t' ol nialeiial. This may be compared to the 

 many sections fiom a (ompleie loal of bread, each slice ha\ing a 



