60 Botanical Microtechnique 



The principal botanical uses for the common stains are indicated 

 in the following tabulation: 



Cellulose cell walls. 



Hematoxylin (self-mordanting type) . 



Fast green FCF. 



Aniline blue. 



Bismarck broAvn Y. 



Acid fuchsin. 

 Lignified cell walls. 



Safranin. 



Crystal violet. 

 Cutinized cell walls. 



Safranin. 



Crystal violet. 



Erytbrosin. 

 Middle lamella. 



Iron hematoxylin. 



Ruthenium red (material cut fresh) . 

 Chromosomes. 



Iron hematoxylin. 



Safranin. 



Crystal violet. 



Carmine (for acetocarmine smears) . 

 Mitochondria. 



Iron hematoxylin. 

 Achromatic figure. 



Crystal violet. 



Fast green FCF. 

 Filamentous fungi in liost tissues. 



Iron hematoxylin. 



Safranin (). 



Fast green FCF. 

 Cytoplasm. 



Eosin Y. 



Erytbrosin B. 



Fast green FCF. 



Orange G or gold orange. 



The above tabulations indicate some relationshii) between the 

 acid or l)asi( character of a stain and its specificity. A basic stain is 

 one in wbicb the (olor bancr is a basic radical; in an acid stain the 

 color bearer is an acid radical. As a rule basic stains are selective for 

 nuclear structtnes and. in sonic processes, lor lignified cell wall. Acid 

 stains usually are selective h)r components ol the c\toplasni and for 

 iMilignified cell wall. 



The common (baring oils (doxe oil. cedar oil, bergamot oil, and 

 wintergreen oil) usualh aie used in (oncentraled h)ini as j)urchased 



