64 Botanical Microtechnique 



STAINING CHART I 



Progressive Hemalum 



Xylene 

 2-5 mill, 

 (de-waxing) 



i 

 absolute 



(anhydrous) 



alcohol 



2-5 min. 



i 

 95% 

 alcohol 

 2-5 min. 



i 



70% 



alcohol 

 2-5 min. 



I 

 50% 



alcohol 



2-5 min. 



i 

 30% 

 alcohol 

 2-5 min. 



i 

 distilled 

 water 

 1-2 min. 



i 

 Hemalum 



5-'^0 min. 



i 

 distilled 



Wilier 



1 min. 



Tap water 



(see page 62) 



resin and 

 cover glass 



t 

 xylene III 



5 min. 



t 

 xylene II 



5 min. 



t 

 xylene I 



5 min. 



T 



carbol- 

 xylene 

 5-10 min. 



t 

 absolute 



alcohol II 



5-10 min. 



absolute 

 alcohol I 

 5-10 min. 



t 

 95% 

 alcohol 

 5-10 min. 



t 



70% 



alcohol 

 5-10 min. 



t 



50% 



alcohol 

 5-10 min. 



t 

 30%) 



alcohol 



2-5 min. 



* 'I he iHKiiiiicr is advised to topv each staiiiiiis chart on a hiiRC card. Bv means of colored 

 arrows, indicate the sequence of operations used to correct overstaining or undcrstainmg. 



or %-in. cover glas.s. For large longitudinal sections of rectangular 

 outline, or for covering several rows of sections on a slide, use a 

 square or long cover glass of such size that there is a margin ol at 

 least 5 mm. Calijicr all cover glasses, using onlv those that lull within 

 0.15 to 0.20 mm. in thickness. 



Canada balsam has been the most widely used mounting medium 



