98 Botanical Microtechnique 



When dissolved, dilute with an equal volume of water. Plate the speci- 

 men on a slide, drain or blot off excess water, put on just enough reagent to 

 cover the material, and heat with a small flame. Proteins give a brick-red 

 color. This is not a highly satisfactory reagent. Futhcrmore, it is highly 

 corrosive and must be used with care. Do not permit inexperienced students 

 to use this reagent on a microscope. The instructor should set up a 

 demonstration microscope, after draining excess reagent frcmi under the cover 

 glass. 



Fats and Oils; Sudan III 



Alcohol (80%) 100 cc. 



Sudan III 0.5 g. 



Cut very thin sections or smear a fragment of the material on a slide, flood 

 with the dye, and cover with a cover glass. After 10 to 20 min. the microscopic 

 globules of fat should assume the bright, clear color of the dye. Cotyledons of 

 the soybean and peanut are good subjects. 



