7 74 Botanical Microtechnique 







Fi(.. 1 1.3— C()iii|);ii isc.ii of fixalion in \()iing oxiilcs of f. ilium: a. I.. sl>cri().siiiii . chroiiie- 



acftic (().5-0.'i) IS hr., akoluilwlciie: h. L. ti<j;yi>iiini . Allcn-Bouin III, 1 davs. ihice- 



gradc (lioxan; c, L. speciosiDii. cliromc-arelic (0.3-0.")) IS In., aholiol-wiciK': </, L. 



tigriiuini. Alk'ii-Udiiin lll.li iiHiiiilis, accuuic-tc'i tiai \ l)iil\l alcoliol. 



inciUs .show \crv link' distorlion. Main ifatlcr.s will iccosiiii/c an old 

 acquaintance in Fig. 1 1.1 h. a t\|)i(ai Bouin, alcohol-xylcnc image that 

 is frecjLiently encountered in lih oxide. .Miliough some batches pre- 

 pared by this method have some good sporocytes, severe plasmolysis 

 <!(( uis iit(|iicnll\ . Ho\\t\ci, nudcai llxalion is iisualh ixitlknl. 



Figure 11.4 c shows \er\ ncarb pciic(i |)icsc r\ at ion ol ihe sporo- 

 cyte. 'Fhe cytoplasm in l<"ig. II. I d is somewhat (oarsely granulai, l)ut 

 the cliromosomes at the beginning oi disjunction in the first division 

 ol meiosis are well lixed. Note that each dnomosome is long and 

 U-shaped, instead ol a (oiiipaci lump as xviili iiikrior fixation. 



