128 Botanical Microtechnique 



0-; 





Fig. 13.4— Growing poinis of .stems: <i. /.ca mays, set'clliiig 1 week old, Craf I, ace- 



tone-xylciic; h, Soja max, seedling 1 week old, Craf II, three-grade dioxan; c, Acer 



saccliariuum. d()nn;nit hud from huge tree, FAA, alcohol wlcne. 



lliiid j)r()iiiiJtly. and pump vigorously. iAA pencil aics well and gives 

 acceptable fixation (Fig. 1.8.4 c). 11 more perfect preservation of the 

 protoplast is desired, dissect away most ol ilu larger leaves, and kill 

 the remaining growing point and small leal j)rimordia in Cral II. 

 Dehydrate the more hiiitle buds in butvl alcohol. Use any of the 

 stains recommended lor pre\ioiis growing points. 



Fi(.. 13.')— Sirm of 7r(i: a. set tor: h. single Ixuidic near periphery of stem. 



