APPENDIX 99 



3. Inorganic Media. (H. C. Bold) 



A. Beijerinck's Solution (Zent. Bakt. Par. Infek. II, 4: 785-787. 

 1898). 



Grams 

 Compound per Liter 



Distilled Water 1000.0 ml 



NH4NO3 0.5 



K2HPO4 0.2 



MgS04.7H20 0.2 



CaCl2.2H20 0.1 



B. Modified Bristol's Solution (Bold, Bull. Torr. Bot. Club, 76: 

 101-108. 1949). 



This solution is prepared as follows: six stock solutions, 400 

 ml. in volume are employed, each containing one of the follow- 

 ing salts in the concentration listed: 



NaNOa 10 g. 



CaCla 1.0 



K2HPO4 3.0 



KH2PO4 7.0 



MgS04 3.0 



NaCl 1.0 



10 ml. of each stock solution are added to 940.0 ml. of Pyrex- 

 distilled water. To this are added also one drop of a 1.0 per cent 

 FeClg solution and 2 ml. of minor elements solution (Trelease 

 and Trelease, Amer. Jour. Bot., 22: 520-542. 1935). 



