Guilliermond - Atkinson 



62 — 



Cytoplasm 



particular that of Agaricus campestris (Beauverie, Guilliermond, 

 Sarazin) and in Coprinus fimetarius (Miss DUCHAUSSOY). All 

 the hyphae which compose the plectenchyma of the foot and of the 

 cap of the sporophore have a chondriome almost exclusively made 

 up of long chondrioconts. In young basidia, before and after nu- 

 clear fusion, there is also found a large number of chondrioconts 

 lying parallel to the long axis of the basidium. As in the case of 

 the asci, the chondrioconts frequently show small vesiculate swell- 

 ings on their long axes. When the two nuclear divisions of the 

 basidium are completed, the chondrioconts fragment into short 

 rods and migrate with the cytoplasm and nuclei to the basidiospores, 

 each of which encloses a chondriome formed of numerous rods. In 

 the course of germination, these rods move into the germinating 

 tube where they multiply and elongate and become long chondrio- 

 conts in the primary mycelium. 



Fig. 27 (left). — Development of the chondriome in Leptomitus. 1, chondrioconts in the 

 vegetative filament: 2, 3. fragmentation of the chondrioconts and grouping about the nuclei 

 during the formation of the zoospores; 4, granular chondriosomes in the zoospores; 5-7, ger- 

 mination of the zoospores, elongation of chondriosomes into chondrioconts. C. chondriocont. 

 N, nucleus. Meves' method, stained with acid fuchsin. 



Fig. 28 (right). — Development of the chondriome in the ascus of Pustularia vesiculosa. 

 1. very young ascus after nuclear division; 2-4, vesiculation of the chondriosomes during 

 growth of the ascus. 5. first mitosis; 6, fragment showing clear zone about each nucleus 

 corresponding to the aster; 7, formation of the ascospores; 8, young ascospores; 9, older 

 ascospores. C, centrosome; N, nucleus. Meves' method. 



We have been able to observe the chondriome in some living 

 fungi: Endomyces Magnusii, Saccharomy codes Ludwigii but the 

 Saprolegniaceae are particularly favorable for this type of study, 

 as we have shown in our research. Meyer as early as 1904 ob- 

 served living chondriosomes in Achlya and described them as leuco- 

 plasts. We have since been able to follow with very great clearness 

 the entire development of the chondriome from the germination of 

 the zoospores to the formation of the zoosporangia in several living 

 fungi of this group: Saprolegnia, Achlya, Leptomitus. The sexual 

 cycle has not been studied as sexual reproduction is not easy to 

 obtain in culture (Guilliermond). 



This study has confirmed results obtained with mitochondrial 

 technique and has thus very emphatically shown that the chondrio- 



