Chapter XVII 



— 195 



Golgi Apparatus 



As a result of these investigations, we extended our research 

 to cover a large number of plants, belonging to the most varied 

 groups, which confirmed and completed our earlier findings. The 

 study of the vacuolar system, notably in the seedling of the pea, 

 gave us particularly suggestive results. In the meristem cells of 

 the root (Fig. 130), there is obtained by silver methods an entirely 

 characteristic reticulate apparatus and it is observed that during 



Fig. 132. — Saprolegnia. Vacuolar system. 1-3, vitally 



stained with neutral red; reticulate, tending to fuse into a 

 vacuolar canal. 4-6, Bensley's method; system appears as 

 canaliculi of Holmgren. 7, 8, da Fano's silver impregnation 

 method; system impregnated with silver, resembles Golgi's 

 network. 9, 10, Kolatchev's osmic impregnation method: 

 system strongly blackened. 



cellular differentiation this network swells and is transformed into 

 small spherical vacuoles, each containing a precipitate heavily 

 blackened by the silver and often arranged as a crescent on the bor- 

 der of the vacuoles, thus appearing like the Golgi elements or dic- 

 tyosomes. Finally, in differentiated regions, the vacuoles are seen 

 to swell and run together to form large vacuoles containing a more 

 or less large number of corpuscles, blackened by a deposit of metal- 

 lic silver. There are, therefore, obtained with silver methods, 

 images which can be perfectly superimposed on those furnished by 

 vital staining with neutral red. 



By means of these methods of impregnation, metallic silver is 

 deposited on the filamentous and reticulate elements of the vacu- 



