Guilliermond - Atkinson 



196 — 



Cytoplasm 



olar system, which gives them a homogeneous black coloration, and 

 in the vacuoles derived by hydration of these elements, the same 

 methods bring about the precipitation of the colloidal contents 

 in the form of corpuscles on which the metallic silver is deposited. 

 The silver methods also permitted us to bring out aleurone 

 grains during their transformation into vacuoles which swell, set 

 first assuming filamentous forms having a tendency to anastomose, 



Fig. 133. — 1-6, Saccharomyces ellipsoideus. 7, Ashbya gossypii. 8-10, Saccharomyces 

 pastorianus. 1-10. da Fano's method; Metachromatin bodies, produced by flocculation from 

 the colloidal solution in the vacuole, strongly blackened by a deposit of metallic silver on 

 their surfaces. 11-18, Geotrichum lactis; capricious impregnation with Kolatchev's method. 

 11, 13, metachromatin bodies within vacuole. 12, 16, 17, metachromatin in vacuoles (v) : 

 chondriosomes (c) are swollen and vesiculated. 14, 15, chondriosomes are swollen and 

 vesiculated. 18, chondriosomes are well preserved. 19-22, Saccharomyces pastorianus; 

 Kolatchev's method; metachromatin bodies blackened by osmium. 



then later appearing as large spherical vacuoles containing nu- 

 merous corpuscles which take up the silver. 



Bensley's method, applied to the meristem cells of the root, 

 brings out canaliculi entirely reminiscent of those of Holmgren. 

 In differentiated regions of the root, these are gradually trans- 

 formed into large vacuoles. 



Results just as diagrammatic were obtained in Saprolegnia. 

 In the extremities of the filaments, Bensley's method produced 

 reticulate figures of the vacuolar system in the form of the appa- 

 ratus of Holmgren and silver methods gave them the appearance 

 characteristic of the reticulate apparatus of Golgi. Farther away 



