EXPERIMENTAL EMBRYOLOGY 



379 



violacea. He cut off the style of one flower and glued it with gelatin 

 to the ovary of another flower whose style had been previously 

 cut away. In order to prevent the falling apart of the grafted style, 

 he supported it against an iron wire, tying the support and the style 

 with a spider's thread (Fig. 2037). When the operation was suc- 

 cessful, the grafted style grew normally and attained its usual size. 



<r? 



■ w 



k-l-- 



£k 



// 



Fig. 203. Diagrams showing technique of splicing (A-H) and grafting (/) of 

 styles, (kk = level at which transverse cut is made through flower bud; s = 

 grafted style; w = wire support; t = spider's thread; o = ovary; g = gelatin joint 

 between ovary and style; p = pedicel). (A-H, after Buchholz, Doak and Blakeslee, 

 1932; I, after Yasuda, 1931.) 



On the other hand, if the tissues failed to unite, the style turned 

 brown and shrank. 



Yasuda 's method requires a great deal of manipulative skill and 

 has apparently never been tried by any other worker. Although it 

 seems to be impracticable with plants having thin styles, it is 

 possible that it can be used successfully when the styles are reason- 

 ably thick. A grafting of the style of the species used as the pa- 

 ternal parent on to the ovary of the maternal parent certainly 

 seems to be a promising method of combating incompatibility fac- 

 tors between two species or varieties. 



