EPHELOTA, GREGARINA 31 



a budding specimen is found keep it well supplied with water 

 and watch for the escape of the buds as free-swimming 

 ciliated embryos. 



3. Fix, stain, and mount in balsam a piece of hydroid with 

 many Ephelota attached. Under the high power note the 

 character of the macronucleus and its relation to the buds. 

 Are micronuclei visible? 



4. Examine carefully the relation of the stalk to the cell 

 body. Compare with that of Vorticella. 



If the material is available study Podophrya and allied 

 forms, with particular reference to the method of budding. 



Collin: Etude monographique sur les Acinetiens. Arch. Zool. Exp. et 

 Gen., 1911 and 1912. 



Root: Reproduction and Reactions to Food in the Suctorian, Podo- 

 phrya Collini, n. sp. Arch. f. Protistenk., 35, 1914. 



SPOROZOA 



GREGARINA 



Remove the head and posterior end of a larval or adult 

 meal beetle and pull out the digestive tract with a pair of 

 forceps. Place the digestive tract on a slide, split it open 

 lengthwise with a sharp scalpel, and then spread it out, with 

 the inner wall exposed, and cover. The operation should be 

 performed rapidly to prevent the material from drying. If 

 the beetle is infected, numerous gregarines will be visible 

 under the microscope. Study with low and high powers. 



1. Does the animal move? A great number of refractive 

 granules are present in the protoplasm. They are regarded 

 as reserve nourishment. They can be removed with acid. 



2. Note that the body is covered with a membrane, and 

 is divided into a dense superficial layer, the ectoplasm, and 

 a central, more fluid mass, the endoplasm. 



3. The endoplasm is separated into two parts by a por- 

 tion of the ectoplasm. The anterior part is termed the pro- 

 tomerite, and the posterior part the deutomerite. In which 

 is the nucleus situated? 



