LOLIGO 159 



Open the spermatophoric sac and remove some of the 

 spermatophores. If the specimen has not been preserved 

 place them immediately in 10 per cent formalin or stronger 

 to keep them from ejaculating. Mount under a cover and 

 examine. The specimens may be stained with dilute Ehrlich's 

 triacid stain and mounted in glycerin jelly if preferred. 

 Notice : 



1. The spermatophore is covered by a transparent elastic 

 outer tunic which has a cap and cap thread at the smaller, 

 oral end. 



2. Inside this is the somewhat granular middle tunic, 

 which is much thicker at the aboral end. 



3. The contents consist of the aboral sperm mass, the oral 

 ejaculatory apparatus, and the cement body, which lies be- 

 tween them. 



4. The sperm mass may be seen to consist of a closely 

 wound thread. It is actually covered by a very thin inner 

 tunic which is separated from the outer tunic by a space filled 

 with liquid. 



5. The cement body is attached to the sperm mass by a 

 narrow core and is covered by a continuation of the inner 

 tunic. It is flask shaped, with the narrow end pointing 

 orally. 



6. The neck of the cement body is covered by parts of the 

 ejaculatory apparatus and the bulge of the body is joined 

 and possibly covered by the outer membrane, which with the 

 inner tunic forms the outer covering of the ejaculatory ap- 

 paratus that appears as a double membrane. Inside the outer 

 membrane is the rather thick middle membrane, the aboral 

 end of which encloses the neck of the cement body and ends 

 against the bulge of the cement body, and the oral end of 

 which is thrown into bends and loops and is finally attached 

 to the cap end of the outer case. Inside the middle membrane 

 is the thin inner membrane and the spiral filament which en- 

 close a narrow lumen. 



7. In ejaculating, the ejaculatory apparatus turns wrong 



