THE ASPERGILLUS GLAUCUS GROUP 121 



Aspergillus chevalieri (Mangin) var. intermedins Thorn and Raper, in U. S. 

 D. A. Misc. Publ. No. 426, p. 21. 1941. 



Colonies upon Czapek's solution agar with 20 percent sucrose differing 

 from the species in texture and color, and presenting withal a picture 

 intermediate between A. chevalieri and .4. amstelodami (fig. 31 B). Asco- 

 spores lenticular, mostly 4.6 to 5.2m by 3.6 to 4.0m, occasionally 5.4m in 

 long axis, with walls roughened and with prominent equatorial crests. 

 Conidiai heads dull green, radiate to columnar, mostly 100 to 125m in 

 diameter, and up to 175m in length ; conidia elliptical to subglobose, smooth- 

 walled, mostly 3 to 4m in long axis. 



Represented in this study by culture NRRL Xo. 82 which was received 

 from George Smith as No. 107 and bore the following notation: "Isolated 

 G. S. from cotton yarn, 1927. Close to .4. chevalieri— differs in having 

 smooth, small conidia and ascospores somewhat larger than type." Dupli- 

 cated by three additional strains received from European sources. 



Aspergillus chevalieri var. intermedins appears to be transitional between 

 the A. chevalieri and the A. amstelodami series. Such a view is supported 

 (1) by the pattern of the ascospores, which shows both the extended and 

 often recurved equatorial crests characteristic of .4. chevalieri and the 

 rough spore walls of A. amstelodami; and (2) by the coloration of the 

 colony. Aspergillus chevalieri var. intermedins upon 20 percent sucrose 

 Czapek agar becomes orange-yellow above and orange to light brown in 

 reverse, A. amstelodami remains bright yellow with reverse uncolored, 

 whereas A. chevalieri becomes red in the colony and reverse. The smooth- 

 ness of conidia in A. chevalieri var. intermedins is a distinctive character and 

 appears in neither A . chevalieri nor A . amstelodami. Although this variety 

 from many points of view appears to be a hybrid, proof of such origin is 

 lacking. 



Aspergillus diplocystis (Sartory, Sartory, Hufschmitt and Meyer) Dodge, Med. 

 Myc. p. 625. 1935. Syn. Eurotium diplocyste Sartory, Sartory, Hufschmitt and 

 Meyer, in Compt. Rend. Soc. Biol. 104: 881-883. 1930. Not E. diplocystis B. and 

 Br., Jour. Linn. Soc. 14: 55-56 Tab. 10. 1875. 



Characterization: Colonies greenish-yellow, becoming yellow from perithecia. 

 Conidiophores erect , 50 to 100m high, 3.1 to 3.7m in diameter, membrane thick, hyaline. 

 Sterigmata confined to a portion of head, 5 to 6.25m by 1.5 to 2.5m- Secondary sterig- 

 mata small ; conidia spherical, 2.25 to 3.1m in diameter, slightly ellipsoid, green (tendre 

 to cendre); sterigmata sometimes abortive and proliferous. Perithecia canary 

 yellow asci 4 to 6m by 5 to 7m, containing 8 ascospores which are ovoid, with a furrow 

 and two crests, 1.5 to 2.5m by 1.8 to 3.1m- 



This description suggests an Aspergillus with the heads approximating .4 . nidulans 

 and the perithecia of A. chevalieri. Ascospore measurements as reported are appre- 

 ciably smaller than those of A. chevalieri or any other known species of Aspergillus. 

 It was described from a case of onychomycosis from the thumb and the great toe. 

 Tentatively placed in the A. chevalieri series. The name is invalid because of E. 

 diplocystis B. and Br. 1S75. 



