160 A MANUAL OF THE ASPP^RGILLI 



Aspergillus quadrilincatus Thorn and Raper. Mycologia 31: 6G0, figs. 2- 



4. 1939. 



Colonies on Czapek's solution agar spreading, plane or slightly wrinkled, 

 with tendency toward floccosity, central area gray with a definite purplish 

 tinge, and olive-green conidial areas toward the margin, occasionally as 

 sectors; perithecia developing separately but abundantly throughout the 

 colony; reverse purplish-red; heads short columnar, green, mostly 60 to 70/z 

 by 30 to 35m, occasionally larger or smaller; conidiophores sinuate, smooth- 

 walled, dull brownish in color, 50 to 75/i in length by 3.5 to 4.5m wide, 

 broadenng to 7.5 to 9/x at the hemispherical vesicular areas; primary 

 sterigmata 5 to 6/x by 2 to 3m, secondary sterigmata 5 to 7 m by 2 to 2.5m; 

 conidia globose, pale yellow-green, rugulose, 3 to 4m in diameter; perithecia 

 enveloped by hiille cells, light brownish in color, spherical, partially em- 

 bedded in the mycelial felt (fig. 42 D), about 125 to 150m in diameter includ- 

 ing the enveloping hiille-cell layer, with perithecial wall 1-cell layer in 

 thickness, ripening quickly and with ripe asci breaking down to leave the 

 ascopores free; ascospores purple-red, lenticular, with smooth wall, with 

 spore body 4 to 4.8m by 3.4 to 3.8m, and with two pleated equatorial crests 

 about 0.5m in width paralleled by a secondary narrower pair (fig. 43 B) 

 which are sometimes indistinct. 



Type NRRL No. 201 (Thorn No. 4138.N8) from New Jersey soil and kept 

 in culture since 1916. Other strains examined include isolations from 

 Texas, Colorado, Louisiana, and Maryland. 



Aspergillus rugulosus Thom and Raper, Mycologia 31: 660-663, 



fig. 4. 1939. 



Colonies on Czapek's solution agar slowly and restricted^ growing (fig. 

 41 C), buckled or wrinkled in a mass 2 to 3 mm. deep, enveloping abundant 

 perithecia at different depths, often eventually splitting in the central area, 

 purple-gray to purple-brown in age, with green heads sparsely produced 

 and hence not generally evident, occasionally seen as small groups and 

 marginal extensions into drying media; reverse in shades of deep purple- 

 red; conidial heads, short columnar, 75 to 100m by 30 to 40m; conidiophores 

 sinuous, smooth-walled, pale brownish in color, 50 to 80m long, slender, 

 varying up to 5m in width, then enlarging to vesicular hemispheres 8 to 10m 

 in diameter; primary sterigmata 7 to 8m by 3 to 3.5m, secondary sterigmata 

 6 to 7m by 2.5 to 3m; conidia globose, green, rugulose, 3 to 4m. 



Perithecia very abundant, often imbedded in the mycelium as 2 or 3 layers 

 and each surrounded by hyphae and dark brown hiille cells (fig. 42 E), 

 globose, 225 to 350m in diameter including mycelial coverings, with dark 

 reddish-purple walls of one cell thickness, quickly ripening and breaking 

 down to leave ascospores free; asci 10 to 11m in long axis; ascospores purple- 

 red, lenticular, walls conspicuously rugulose (fig. 43 C), with spore bodies 4 



