THE ASPERGILLUS FLAVUS-ORYZAE GROUP 263 



The production of perithecia by members of this series was reported by 

 Bezssonoff (1919) without adequate description and by Zikes (1922) whose 

 culture, as received from him, belonged in the A. glaucus group. No 

 ascosporic form is verifiable for the group thus far. 



Aspergillus micro-virido-citrinus Costantin and Lucet, in Ann. Sci. Xat. 



Bot. (IX) 2: 158. 1905. 



The appearance of colonies and measurements of conidiophores, heads, 

 and spores indicate a form intermediate between A. flavus and .4. oryzae 

 except for its small conidia. The description is very nearly satisfied by 

 Takahashi's culture "P" (XRRL Xo. 480). It was found to grow between 

 15° and 45° C. and to be pathogenic to rabbits. Colonies were greenish- 

 yellow to predominantly yellow but contained some definitely green admix- 

 ture in contrast to A. oryzae, which often lacks green color entirely. Co- 

 nidiophores 600 to 1700m in length, up to 21m in diameter near the vesicle, 

 uncolored, "granular" (= pitted) above, smooth toward the base. Vesicles 

 24 to 62m in diameter. Sterigmata varying in size and arrangement with 

 the size of the heads examined. Conidia globose, smooth, 3 to 4.6m (3.1m 

 as a minimum to occasional diameters of 5.5m). 



An occasional culture shows the morphological characters described by 

 Costantin and Lucet. Xo actual identity has been proved. 



Aspergillus flavus Link, in Obs. p. 16. 1809; also in Sp. Plant. 6: 66. 

 1824, cited as synonym of Monilia flava Persoon, Myc. 1, p. 30. 



Synonym: Eurotium Aspergillus flavus DeBary and Woronin, in Beitrage 

 zur Morphologic und Physiologie der Pilze, III Reihe, p. 

 380. 1870. Exsiccati by Brefeld preserved in Rabenhorst, 

 Fungi Europaei Edit. Xov. ser. II, Xo. 2135; one packet 

 in the collection of the Xew York Botanical Garden. 



Colonies on Czapek's solution agar spreading rapidly, with floccosity 

 limited to scanty growth of sterile hyphae in older and dryer areas among 

 crowded conidiophores; conidial areas range in color in various strains from 

 sea-foam yellow through chartreuse yellow, citron green, lime green, to 

 Kronberg's green (PI. VII D and fig. 72 A), or even to ivy green, (See Ridg- 

 way, PI. XXXI, column 25), yellow-green colors are either persistent or, in 

 old colonies, altered by the disappearance of the green factor leaving shades 

 of yellow-brown; reverse yellowish at first, passing over into brown shades 

 in age. Conidial heads vary from small with a few chains of conidia to 

 large radiate (fig. 72 E) or columnar masses in the same culture and varying 

 mixtures of different types and sizes of head. Conidiophores mostly arising 

 from submerged hyphae, commonly 400 to 1000m l°ng by 5 to 15m in diame- 



