PREFACE TO THE SECOND EDITION 



It is gratifying to the author to learn that the kindly reception ac- 

 corded to Methods in Plant Histology has exhausted the edition. Since 

 the first edition appeared, a httle more than four years ago, laboratory 

 methods have been greatly improved, and systematic experiments 

 have made it possible to give much more definite directions for making 

 preparations. 



In the present edition much more attention has been given to col- 

 lecting material. Professor Kleb's methods for securing various repro- 

 ductive phases in the. algae and fungi have been outlined in a practical 

 way. Methods for growing other laboratory material are more com- 

 plete than in the earlier edition. 



The paraffin method has been much improved, and the glycerin 

 method has been almost entirely replaced by the Venetian turpentine 

 method, to which a whole chapter is devoted. Other new chapters 

 deal with microchemical tests, freehand sections, special methods, 

 and the use of the microscope. 



The author is deeply indebted to his colleague, Dr. W. J. G. Land, 

 for numerous suggestions and improvements in methods. 



Corrections and suggestions will be heartily appreciated. 



Charles J. Chamberlain 

 Chicago 



July 1, 1905 



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