PREFACE TO THE THIRD EDITION 



The continued appreciation accorded to Methods in Plant His- 

 tologij has exhausted the second edition. Since that edition appeared, 

 methods have become more and more exact, so that the present vol- 

 ume is practically a new book. The general arrangement of the sub- 

 ject matter, and directions for collecting material and for securing 

 reproductive phases in the algae and fungi have been retained, and a 

 chapter on ''Photomicrographs and Lantern Slides" (chap, xii) has 

 been added. 



Great improvements have been made in the paraffin method, so that 

 sections are easily cut which were impossible ten years ago, while ten 

 years of added experience with the Venetian turpentine method have 

 made it possible to describe it so definitely that even the beginner 

 should find no serious difficulty. 



The author is deeply indebted to his colleague, Dr. W. J. G. Land, 

 for numerous suggestions and improvements covering the whole field 

 of microtechnique. He is also greatly indebted to Dr. S. Yamanouchi 

 for many improvements in the methods applicable to algae and mi- 

 totic figures. 



Corrections and suggestions will be heartily appreciated. 



Charles J. Chamberlain 

 Chicago 

 May, 1915 



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