luji 



A 



PREFACE TO THE FIFTH EDITION 



As one edition of this book has followed another, we have tried to 

 keep the methods up to the highest possible standard. We have tried 

 to keep our own technique up to date and have had the pleasure of 

 seeing various phases of microtechnique as practiced in the leading 

 universities of our own country and also in the laboratories of Eng- 

 land, Europe, and the Orient. 



The directions for collecting material have been still further ampli- 

 fied, and directions for fixing, dehydrating, and staining have been 

 improved. 



"Paleobotanical Microtechnique" and "Laboratory Photography" 

 have been amphfied and given chapter headings. There is a new chap- 

 ter on "Illustrations for Pubhcation." The steam method and 

 Jeffrey's vulcanizing method for sectioning hard woods, and Gourley's 

 method for staining living vascular tissues will repay the time one 

 must spend to gain a mastery of these phases of technique. 



The paraffin method has been improved for both dehcate and hard 

 tissues. Improvements have been made throughout; in fact, the book 

 has had a rewriting, not a mere revision. 



The author is even more deeply indebted than before to his friend 

 and colleague. Dr. W. J. G. Land, for suggestions and improvements 

 covering the entire field of microtechnique and photography. He is also 

 indebted to Dr. S. Yamanouchi for refinements in the paraffin method 

 — from fixing to the finished mount — which make 3 and 2 micron sec- 

 tions easy, 1 micron sections not too difficult, and | micron sections 

 a possibility. Dr. Paul J. Sedgwick has written the parts deahng with 

 "Botanical Photomicrography" and "Movie Photomicrography." 

 To Miss Ethel Thomas, who assisted me for many years, I am in- 

 debted for improvements, criticism, and suggestions covering the 

 entire range of the book. Besides, I must thank a host of colleagues 

 and students all over the world for help in all phases of the subject. 



Corrections and suggestions will be heartily appreciated. 



^ Charles J. Chamberlain 



Chicago 



April, 1932 



IX 



