CONTENTS 



PART I 



PAGE 



Introduction 3 



Chapter I. Apparatus 7 



Chaptkr II. Reagents 18 



Killing and Fixing Agents 18 



The Alcohols 19 



The Chromic- Acid Group 21 



Picric Acid 29 



Corrosive Sublimate 30 



Iodine 32 



Formalin 32 



General Hints on Fixing 33 



Dehydrating Agents 34 



Clearing Agents 37 



Miscellaneous Reagents 40 



Chapter III. Stains and Staining 42 



The Haematoxylins 45 



The Carmines 53 



The Anilins 56 



Double Stains and Triple Stains 65 



Chapter IV. Genera:. Remarks on Staining 72 



Choosing a Stain ' 72 



Theories of Staining 73 



Practical Hints on Staining 77 



Chapter V. Temporary Mounts and Microchemical Tests . . 80 



Temporary Mounts 80 



Microchemical Tests 82 



Chapter VI. Freehand Sections . . •. 87 



Woody and Herbaceous Sections 88 



Objects Mounted without Sectioning 97 



Chapter VII. The Glycerin Method 100 



Chapter VIII. The Venetian Turpentine Method 106 



xi 



^279G 



