xii CONTENTS 



PAGE 



Chapter IX. The Paraffin Method 112 



Killing and Fixing 112 



Washing 114 



Hardening and Dehydrating 115 



Clearing 116 



The Transfer from Clearing Agent to Paraffin 117 



The Paraffin Bath 118 



Imbedding 120 



Cutting 122 



Fixing Sections to the Slide 125 



Removal of the Paraffin 129 



Removal of Xylol or Turpentine 129 



Transfer to the Stain 129 



Dehydrating 129 



Clearing 130 



Mounting in Balsam 130 



A Tentative Schedule for Paraffin Sections 131 



Chapter X. The Celloidin Method 132 



Chapter XL The Cellulose Acetate Method 138 



Chapter XII. Special Methods 140 



Very Large Sections 140 



Stony Tissues 141 



Steam Method for Hardwood Sections 141 



Jeffrey's Vulcanizing Method 142 



Clearing Thick Sections or Small Objects 143 



Land's Gelatin Method 144 



Schultze's Maceration Method 144 



Jeffrey's Maceration Method 145 



Protoplasmic Connections 145 



Staining Cilia 149 



Chondriosomes 149 



Canaliculi 151 



Gourley's Method for Vascular System 151 



Staining Living Structures 152 



Chapter XIII. Paleobotanical Microtechnique 154 



Sections 154 



Peels 157 



