CHLOROPHYCEAE 



237 



formly makes poor preparations. In order to get good preparations of 

 the antheridium it is necessary to disregard other structures, which 

 will be stained lightly or not at all when the stain is just right in the 

 antheridial filaments. 



B 



Fig. 57. — Chara: fixed in chromo-acetic acid and stained in safranin, gentian violet, orange. 

 A, longitudinal section of apex showing five nodes; c, corticating filaments; j;, part of the frag- 

 mented nucleus. B, note the different condition of the nodes and first branches. C, young an- 

 theridium, a, and young oogonium, o. X230. 



If it is desired to mount whole branches showing the antheridium and 

 oogonium in position, use the Venetian turpentine method, staining in 

 phloxine alone, or in phloxine and anilin blue. Good mounts showing 

 shield, manubrium, capitula, and filaments may be obtained by crush- 



